Sterilität beim substrat?

[MR.BLAAA]

Hallo Forum
Ich habe vor einigen Tagen eine petrischale mit Nährboden, mit einer Pilzprobe besetzt und nun zeigt sich das ersten Myzelwachstum (hoffe ich zumindest).
Also bezüglich des myzels wollte ich mich vergewissern ob es sein kann das nach 3 tagen bereits 1,5cm Radius an Myzel sich gebildet hat und dabei leichte Strukturen oberhalb des Bodens zu erkennen sind. (Ist ein roträndiger baumschwamm, nur für ein wissenschaftliches Projekt)

Ich gehe trotzdem von erfolg des Wachstums aus und so stellt sich mir die frage ich mit Substrat agieren soll, ich habe vor Stroh, Hafer und Kaffeesatz als Substrat zu verwenden. Muss ich dieses nun vor „beimpfen“ sterilisieren? Und wenn ja, wie?

Vielen dank schonmal.
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Antworten

[prof lab boy]

Dear Mr. BLAAA

Not to waste time for both of us (maybe it will be):

Question No.1:
Are you familiar with sterile work techniques?

Question No.2:
Do you have any experiences to grow mushrooms under sterile conditions?

You seem to be quite sure that you already occupied the petri dish three days before you wrote your message. So, you occupied the dish on November 12th.
I hope (on your behalf) and hope in your interest you did occupy the d ish.

I’m a little bit confused.
Did you check the distance of mycelial growth between November 12th and November 15th?
It sees as if you suddenly realized first growth on October 15th.

You ask if it is possible to get 15mm of (fresh?) growth radius in three days.
Generally, this is a possible value for a lot of mushroom species under more or less optimal growth conditions.
I’m not sure if that is realistic for the special mushroom species you want to grow at your setup.
According to my information some similar kinds of wood digesting fungi have relative slow growth.

Maybe stupid questions:
How do you measure the radius of the mushroom colony?
Do you measure from the center of your inoculum or from the first fresh growth on the dish?
How big was the sample you used to inoculate the petri dish?
What kind of inoculum did you use?
Is the sample you used as inoculum a clone or spore sample directly from a fresh mushroom?
So, did the tissue or mycelium have to reorganize first?
Or did spores first need to geminate?
Was your sample already isolated before and you just reactivated mycelium?
Was your sample growing on comparable nutrients before?
So, was the mycelium adapted to the given nutrients on your dish?

Further philosophic interactions don’t make any sense before you know you can get a clean colony and tell more about your setup and your state of expertise.

Just one more question so far:
Why do you want to grow a wood loving species on straw?